Jing Chen, MD, PhD
Assistant Scientist
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Dept. of Pathology, Immunology and Laboratory Medicine |
Currently Involved In:
To understand the genetic basis of human Type 1 diabetes (T1D), I am using mouse models to define the mechanism specific genes play in disease susceptibility and resistance. Previously we have identified a gene, encoded within the mitochondrial genome, that is protective against T1D in both humans and mice. This gene changes mitochondrial function. Mitochondria are essential for the life and death of cells, and free radicals produced by these organelles modulate cellular functions. One of my major goals is to understand how mitochondria affect two important cellular players in T1D, T cells and beta cells. My second goal is to elucidate the interactions occurring between the nuclear and mitochondrial genomes. The cross talk between these two genetic compartments is likely essential for controlling free radical production and cellular function of human beta cells as well as activation of immune cells.
Publications
- Chen J, Grieshaber S, Mathews CE (2011). Methods to assess beta cell death mediated by cytotoxic T lymphocytes. J Vis Exp. Jun 16;(52). pii: 2724. doi: 10.3791/2724.
- Chen J, Gusdon AM, Mathews CE (2011). Role of genetics in resistance to type 1 diabetes. Diabetes/Metabolism Research & Review. In press
- Lightfoot YL, Chen J, Mathews CE (2011). Role of the Mitochondria in Immune-Mediated Apoptotic Death of the Human Pancreatic A Cell Line ALox5. PLoS One. 6(6):e20617. Epub 2011 Jun 27.
- Thayer TC, Delano M, Liu C, Chen J, Padgett LE, Tse HM, Annamali M, Piganelli JD, Moldawer LL, Mathews CE (2011). Superoxide Production by Macrophages and T Cells Is Critical for the Induction of Autoreactivity and Type 1 Diabetes. Diabetes. Jun 29. [Epub ahead of print]
- Chen J, Gusdon AM, Piganelli JD, Leiter EH, Mathews CE (2011). mt-Nd2a modifies resistance against autoimmune Type 1 diabetes in NOD mice at the level of the pancreatic beta cell. Diabetes. 60(1):355-9.
- Li J, Liu X, Ran X, Chen J, Li X, Wu W, Huang H, Huang H, Long Y, Liang J, Cheng J, Tian H. (2010). Sterol regulatory element-binding protein-1c knockdown protected INS-1E cells from lipotoxicity. Diabetes Obes Metab. 12(1):35-46
- Gusdon AM, Chen J, Votyakova TV, Mathews CE (2009). Quantification, localization, and tissue specificities of mouse mitochondrial reactive oxygen species production. Methods Enzymol. 456:439-57.
- Chen J, Gusdon AM, Thayer TC, Mathews CE (2008). Role of increased ROS dissipation in prevention of T1D: Lessons from the ALR mouse. Ann N Y Acad Sci. Dec; 1150:157-66.
- Zhang M, Chen J, Guo Z, Li L, Zhang Y, Li J (2009). Rosiglitazone inhibits RANTES expression by human epidermal keratinocytes. Eur J Dermatol. Mar 1;19(2):176-177.
- Chen J, Lu Y, Lee C-H, Li RH, Leiter EH, Mathews CE (2008). Comparative Genetics of Spontaneous Autoimmune versus Chemically-Induced Diabetes in Mice. Free Radic Biol Med. Nov 1;45(9):1263-70
- Chen J, Hatori, Y, Nakajima K, Eizawa T, Ehara T, Koyama M, Hirai T, Fukuda Y, Kinoshita M, Suguyama A, Hayashi J-I, Onaya T, Kobayashi T, Tawata M(2006). Mitochondrial complex I activity is significantly decreased in a patient with maternally inherited type 2 diabetes mellitus and hypertrophic cardiomyopathy associated with mitochondrial DNA C3310T mutation: a cybrid study. Diabetes Res Clin Pract. Nov;74(2):148-53
- Chen J, Chen YG, Reifsnyder PC, Schott WH, Lee CH, Osborne M, Scheuplein F, Haag F, Koch-Nolte F, Serreze DV, Leiter EH (2006). Targeted disruption of CD38 accelerates autoimmune diabetes in NOD/Lt mice by enhancing autoimmunity in an ADP-ribosyltransferase 2-dependent fashion. J Immunol. Apr 15;176(8):4590-9.
- Lee CH, Chen YG, Chen J, Reifsnyder PC, Serreze DV, Clare-Salzler M, Rodriguez M, Wasserfall C, Atkinson MA, Leiter EH(2006). Novel leptin receptor mutation in NOD/LtJ mice suppresses type 1 diabetes progression: II. Immunologic analysis. Diabetes. Jan;55(1):171-8.
- Chen Y-G, Chen J, Osborne MA, Chapman HD, Besra GS, Porcelli SA, Leiter EH, Wilson B, Serreze D (2006) CD38 is required for the peripheral survival of immunotolerogenic CD4+ iNKT-cellsin NOD mice. J Immunol. Sep 1;177(5):2939-47
- Chen J, Reifsnyder PC, Scheuplein F, Schott WH, Mileikovsky M, Soodeen-Karamath S, Nagy A, Dosch HM, Ellis J, Koch-Nolte F, Leitei EH (2005). "Agouti NOD": Identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells" Mammalian Genome. Oct;16(10):775-83
- Lee C-H, Reifsnyder PC, Naggert JK, Chen J, and Leiter EH (2005). Novel Leptin Receptor Mutation in NOD/LtJ Mice Suppresses Type 1 Diabetes Progression. I. Pathophysiologic analysis. Diabetes.Sep;54(9):2525-32.
- Ohkubo E, Aida K, Chen J, Hayashi JI, Isobe K, Tawata M, Onaya T (2000). A patient with type 2 diabetes mellitus associated with mutations in calcium sensing receptor gene and mitochondrial DNA. Biochem Biophys Res Commun. 278(3): 808-13.
- Aida K, Ikegishi Y, Chen J, Tawata M, Ito S, Maeda S, Onaya T (2000). Disruption of aldose reductase gene (Akr1b1) causes defect in urinary concentrating ability and divalent cation homeostasis. Biochem Biophys Res Commun. 277(2):281
- Tawata M, Hayashi JI, Isobe K, Ohkubo E, Ohtaka M, Chen J, Aida K, Onaya T (2000). A new mitochondrial DNA mutation at 14577 T/C is probably a major pathogenic mutation for maternally inherited type 2 diabetes. Diabetes. 49(7):1269-7