Marguerite Hatch, B.Sc., PhD
Professor
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Dept. of Pathology, Immunology and Laboratory Medicine |
Primary Area of Research:
My general interests are focused on the mechanisms and control of electrolyte and solute transport across epithelial membranes. In particular, I am interested in transport mechanisms for the oxalate anion across intestinal and renal epithelial in the context of calcium oxalate kidney stone disease and Primary Hyperoxaluria.
Current investigations in my laboratory are focused on several anion exchangers in the SLC26A gene family that are involved in epithelial oxalate transport. Using a variety of transport physiology and molecular biology approaches and studies that include whole animal (mouse knockout models) and tissue (intestine and kidney) in addition to cell culture studies, we are evaluating the contribution of specific transport proteins to epithelial movements of oxalate. My interest is also focused on the physiological interaction between the gut-resident Oxalobacter formigenes and the enterocyte which results in alterations of intestinal oxalate transport and enteric elimination of oxalate leading to reduced renal excretion of this stone-forming compound.
Related Links:
http://www.ohf.org/
http://www.pathology.ufl.edu/~freel/
Publications
- Hatch, M., Gjymishka, A., Salido, E.C., Allison, M.J., and Freel, R.W. Enteric oxalate elimination is induced and oxalate is normalized in a mouse model of Primary Hyperoxaluria following intestinal colonization with Oxalobacter. 2011. Am. J. Physiol. Gastrointest. Liver Physiol. 300: G461-G469.
- Li, N., Hatch, M., Wasserfall, C., Douglas-Escobar, M., Atkinson, M., Schatz, D., and Neu, J. Butyrate and Type 1 Diabetes: Can We Fix the Intestinal Leak? 2010. J. Ped. Gastro. & Nutr.
- Freel, R.W., Morozumi, M., and Hatch, M. 2009. Parsing apical oxalate exchange in Caco-2BBe1 monolayers: siRNA knockdown of SLC26a6 reveals the role and properties of PAT-1. Am. J. Physiol. Gastrointest. Liver Physiol. 297: G918-G929.
- Hatch, M., and Freel, R.W. Increased colonic sodium absorption in rats with chronic renal failure is partially mediated by AT1 receptor agonism. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295: G348-G356.
- Freel, R.W., and Hatch, M. Enteric oxalate secretion is not directly mediated by the human CFTR chloride channel. 2008. Urol Res. 36: 127-131.
- Hatch, M., and Freel, R.W. 2008. The roles and mechanisms of intestinal oxalate transport and oxalate homeostasis. Sem in Neph. 28 #2:143-151.
- Hatch, M., Cornelius, J., Allison, M., Sidhu, H., Peck, A, and Freel, R.W. 2006. Oxalobacter sp. reduces urinary oxalate excretion by promoting enteric oxalate secretion. Kid. Int. 69 #4: 691-8.
- Freel, R.W., Hatch, M., Green, M., and Soleimani, M. 2006. Ileal oxalate absorption and urinary oxalate excretion are enhanced in slc26a6-null mice. Amer. J. Physiol. (Gastro.): 290 #4:G716-28.
- Green, M., and Freel, R.W., and Hatch, M. 2005. Lipid peroxidation is not the underlying cause of renal injury in hyperoxaluric rats. Kid. Int. 68 #6: 2629-2638.
- Green, M., Hatch, M., and Freel, R.W. 2005. Ethylene Glycol Induces Hyperoxaluria Without Metabolic Acidosis in Rats. Am. J. Physiol. (Renal) 289 #3: F536-F543.
- Neu, J., Reverte, C. M., Mackey, A. D., Liboni, K., Tuhacek-Tenace, L. M., Hatch, M., et al. 2005. Changes in intestinal morphology and permeability in the biobreeding rat before the onset of type 1 diabetes. J. Pediatr. Gastroenterol. Nutr. 40 (#5): 589-95
- Hatch, M. and Freel, R.W. 2005. Intestinal transport of an obdurate anion: oxalate. Urol. Res. 33: 1-16.
- Morozumi, M, Green, M., Freel, R.W. and Hatch, M. 2004. The effect of oxalate loading or acidified media on the expression of mRNA encoding candidate oxalate transporters. Proceedings of the 10th International Symposium on Urolithiasis in Hong Kong pp 178-180.
- Harris, A., Freel, R.W, and Hatch, M. 2004. Serum oxalate concentrations in rats and humans as determined with the use of ion chromatography. J.Lab.Clin. Med. 144, #1, 45-52.
- Hatch, M. and Freel, R.W. 2003. Angiotensin II Involvement in Enteric Oxalate Excretion in Rats with Chronic Renal Failure Induced by Hyperoxaluria. Urol. Res. 31: 426-432.
- Hatch, M. and Freel, R.W. 2002. Renal and intestinal handling of oxalate following oxalate loading in rats. Am. J. Nephrol. 23, #1, 18-26.