Test Menu


Please make sure you read the DRL test policies and procedures before ordering any tests.

Test List:

1p36/19q13 Fluorescence in situ hybridization  (Yachnis, Anthony T., MD)

  Test Order Number:0295
  Methodology:FISH
  Specimen Requirements:Formalin Fixed Paraffin embedded tissue
  Specimen Handling:room temperature
  In-house turnaround time:9 days
  Reference Values:Non deleted
  CPT Code:88365 x 4

1p36/19q13 Fluorescence in situ hybridization (FISH)  (Anthony Yachnis M.D.)

  Test Order Number:0295
  Methodology:FISH
  Specimen Requirements:Formalin Fixed Paraffin embedded tissue
  Specimen Handling:Submitt block at room temperature.
  In-house turnaround time:9 days
  Reference Values:Non deleted
  CPT Code:88365 x 4

Adrenocortical Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0022
  Methodology:Indirect immunofluorescence
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:Negative (Interpretive report provided)
  CPT Code:88347

Anti-B2 Glycoprotein I  (Dr. Neil Harris)

  Test Order Number:0229
  Methodology:ELISA
  Specimen Requirements:5 ml of blood collected in sodium citrate (blue top) by normal non-traumatic venipuncture.
  Specimen Handling:Seperate plasma by centrifugation at 1500g for 15 min. at 4C. Ship frozen.
  In-house turnaround time:10 days
  Reference Values:IgG <20U/mL IgM <10U/mL
  CPT Code:86146 x 2

Anti-Cardiolipin  (Dr. Neil Harris)

  Test Order Number:0032
  Methodology:ELISA
  Specimen Requirements:5 ml of blood collected in sodium citrate (blue top) by normal non-traumatic venipuncture.
  Specimen Handling:Seperate plasma by centrifugation at 1500g for 15 min. at 4C. Ship frozen.
  In-house turnaround time:10 days
  Reference Values:IgG <11 GPL U/mL IgM <10 MPL U/mL
  CPT Code:86147 x 2

Antiphosphotidylserine (PS)  (Dr. Neil Harris)

  Test Order Number:0114
  Methodology:ELISA
  Specimen Requirements:5 ml of blood collected in sodium citrate (blue top) by normal non-traumatic venipuncture.
  Specimen Handling:Seperate plasma by centrifugation at 1500g for 15 min. at 4C. Ship frozen.
  In-house turnaround time:10 days
  Reference Values:IgG <11.0 GPS U/mL IgM <25.0 MPS U/mL
  CPT Code:86148 x 2

BCL-2 t(14:18) Translocations  (Li, Ying, MD, PhD)

  Test Order Number:0248
  Methodology:PCR, Southern blot
  Specimen Requirements:Tissue, cell suspension, peripheral blood in EDTA
  Specimen Handling:Ship blood or bone marrow specimens at 2-8oC. Ship Formalin fixed and/or paraffin embedded samples at room temperature.
  In-house turnaround time:9 days
  Reference Values:not detected
  CPT Code:83907, 83891, 83898x2, 83894x2, 83897x2, 83896x2,

Breast Pathology, General Info.  (Nicole Massoll, M.D.; Peter A. Drew, M.D.; Christine Orlando, D.O.; Demaretta Rush, M.D.; Samer Al-Quran, M.D.; Lisa Dixon, M.D.)

  Test Order Number:
  Methodology:Quantitative immunohistochemistry of ER, PR, Mib-1 (Ki-67), and HER2, DNA ploidy analysis by flow cytometry, and FISH quantitation of HER2.
  Specimen Requirements:Tissue in appropriate fixative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report, Quantitative immunohistochemistry, DNA ploidy. HER-2/neu analysis.
  CPT Code:

Chlamydia trachomatis and/or Neisseria gonorrhoeae  (Rand, Kenneth H., MD)

  Test Order Number:
  Methodology:Target Amplification Nucleic Acid Probe
  Specimen Requirements:Female endocervical, vaginal or male urethral swabs, urine specimens, Cervical Brush in ThinPrep® transport Pap Test™ Media or SurePath media.
  Specimen Handling:Use Gen-Probe® APTIMA Combo 2 collection and transport kit.
  In-house turnaround time:5 days
  Reference Values:not detected
  CPT Code:87491 CT and 87591 NG

CYTOLOGY, THINPREP PAP TEST  (Nicole Massoll, M.D. Edward J. Wilkinson, M.D. Jacqueline A. Knapik, M.D. Anthony Yachnis, M.D. Demaretta Rush, M.D. )

  Test Order Number:
  Methodology:ThinPrep 2000 System
  Specimen Requirements: This test requires a ThinPrepR PAP TestTM special collection kit that must be ordered separately through DRL customer services at 352-265-9900, or 1-888-375-5227.
  Specimen Handling:Do not use expired kits.
  In-house turnaround time:1 to 3 days
  Reference Values:
  CPT Code:Dependant on Testing Circumstances

Cytopathology, General Info.  (Edward J. Wilkinson, M.D.; Peter A. Drew, M.D.; Jacqueline A. Knapik, M.D.; Nicole Massoll, M.D.; Christine Orlando, D.O.; Demaretta Rush, M.D.)

  Test Order Number:
  Methodology:Light microscopy, immunohistochemistry.
  Specimen Requirements:Pre-prepared slides and/or paraffin block; alcohol fixed smears for non-gynecological specimens.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Dermatopathology, General Info.  (Vladimir Vincek, M.D., Ph.D Frederick L. Glavin, M.D., Vladimir Vincek, M.D., Ph.D )

  Test Order Number:
  Methodology:Light microscopy, immunofluorescence (direct and indirect), electron microscopy, special stains (conventional and immunohistochemistry), flow cytometry, image cytometry.
  Specimen Requirements:Tissue in appropriate fixative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 3 days
  Reference Values:Interpretive report
  CPT Code:

Detection of Chlamydia trachomatis and/or Neisseria gonorrhoeae  (Rand, Kenneth H., MD)

  Test Order Number:CT/NG
  Methodology:Target Amplification Nucleic Acid Probe
  Specimen Requirements:Female endocervical, vaginal or male urethral swabs, urine specimens, Cervical Brush in ThinPrep® transport Pap Test™ Media or SurePath media.
  Specimen Handling:Use Gen-Probe® APTIMA Combo 2 collection and transport kit.
  In-house turnaround time:5 days
  Reference Values:not detected
  CPT Code:87491 CT and 87591 NG

Ear, Nose, Throat (ENT)/Endocrine, General Info.  (Christine Orlando, D.O., Director; Peter Drew, M.D.; Robert Allan, M.D.; Samer Al-Quran, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry)
  Specimen Requirements:Tissue in appropriate fixative/preservative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Endocrine Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0024
  Methodology:Indirect immunofluorescence, Hemagglutination
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery
  In-house turnaround time:10 working days (see Note)
  Reference Values:Negative
  CPT Code:88347 (x6), 86376, 86800

Eye Pathology, General Info.  (Thomas Eskin, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry).
  Specimen Requirements:Tissue in appropriate fixative/preservative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:2 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Factor V Leiden  (Dr. Neil Harris)

  Test Order Number:0206
  Methodology:PCR
  Specimen Requirements:5 mLs of anticoagulated blood in EDTA (purple top) collected by normal non-traumatic venipuncture.
  Specimen Handling:Store and ship at room temperature.
  In-house turnaround time:10 days
  Reference Values:Normal
  CPT Code:83891, 83894 x 2, 83898 x 2, 83912

Gastric Parietal Cell Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0021
  Methodology:Indirect Immunofluorescence
  Specimen Requirements:1-2 mL serum
  Specimen Handling:
  In-house turnaround time:10 working days
  Reference Values:Negative
  CPT Code:88347

Gastrointestinal Pathology/Hepatopathology, General Info.  (Chen Liu, M.D., Ph.D.; James M. Crawford, M.D.; John Reith, M.D.; Lisa Dixon, M.D.; May Arroyo, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry).
  Specimen Requirements:Tissue in appropriate fixative/preservative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Genitourinary/prostate/bladder/Male Gu, General Info.  (Robert Allan, M.D.; Peter Drew, M.D.; Christine Orlando, D.O.; Jacqueline Knapik, M.D.; William Clapp, M.D., Byron Croker, M.D., Ph.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry).
  Specimen Requirements:Tissue in appropriate fixative/preservative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Glutamic Acid Decarboxylase Autoantibody (GAD65)  (William E. Winter, M.D.)

  Test Order Number:0277
  Methodology:RIA
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:< 1.1 u/mL
  CPT Code:86341

Gynecologic Pathology, General Info.  (Nicole A. Massoll, M.D.; Edward J. Wilkinson, M.D.; Peter Drew, M.D.; Jacqueline A. Knapik, M.D.; Anthony Yachnis, M.D.; Demaretta Rush, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry). DNA ploidy analyses and immunohistochemistry for assessment of possible molar pregnancy.
  Specimen Requirements:Tissue in appropriate fixative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report
  CPT Code:

HCV Genotyping LiPA2.0  (Rand, Kenneth H., MD)

  Test Order Number:0814
  Methodology:LiPA 2.0
  Specimen Requirements:1 mL Serum / Plasma EDTA, 4 mL peripheral blood in EDTA
  Specimen Handling:process whole blood within 4 hrs of the collection,store and ship specimen at –60oC to –80oC. Avoid repeated freeze/thaw of the samples.
  In-house turnaround time:5 days
  Reference Values:
  CPT Code:87902

HCV Viral Load bDNA  (Rand, Kenneth H., MD)

  Test Order Number:0804
  Methodology:bDNA
  Specimen Requirements:1 mL Serum / Plasma EDTA, 4 mL peripheral blood in EDTA
  Specimen Handling:process whole blood within 4 hrs of the collection,store and ship specimen at –60oC to –80oC. Avoid repeated freeze/thaw of the samples.
  In-house turnaround time:5 days
  Reference Values:< 615 IU/ mL
  CPT Code:87522

Hematopathology, General Info.  (Raul C. Braylan, M.D.; Lijun Yang, M.D.; Ying Li, M.D., Ph.D.; Samer Al-Quran, M.D.; Robert Allan, M.D.)

  Test Order Number:
  Methodology:Flow cytometry, gene rearrangements by molecular methods, immunohistochemistry.
  Specimen Requirements:Peripheral blood, bone marrow, needle aspirates and serous fluids in appropriate fixatives; Fresh, unfixed tissue and/or lymph nodes.
  Specimen Handling:
  In-house turnaround time:Varies (methodologies dependent)
  Reference Values:Interpretive report with flow cytometry and molecular testing when indicated.
  CPT Code:

Heparin Platelet Antibody  (Dr. Neil Harris)

  Test Order Number:0219
  Methodology:ELISA
  Specimen Requirements:5 ml of blood collected in sodium citrate (blue top) by normal non-traumatic venipuncture.
  Specimen Handling:Seperated plasma by centrifugation at 1500g for 15 min. at 4C. Ship frozen.Seperated plasma by centrifugation at 1500g for 15 min. at 4C. Ship frozen.
  In-house turnaround time:6 days
  Reference Values:<4.0 relative O.D.
  CPT Code:86022

Her2/neu Fluorescence in situ hybridization (FISH)  (Wilkinson, Edward J., MD Massoll, Nicole Ann, MD )

  Test Order Number:0281
  Methodology:FISH
  Specimen Requirements:Formalin Fixed Paraffin embedded tissue
  Specimen Handling:
  In-house turnaround time:9 days
  Reference Values:not amplified
  CPT Code:88368 x 2

HIV-1 Viral Load bDNA  (Rand, Kenneth H., MD)

  Test Order Number:
  Methodology:bDNA
  Specimen Requirements:1 mL Serum / Plasma EDTA, 4 mL peripheral blood in EDTA
  Specimen Handling:process whole blood within 4 hrs of the collection,store and ship specimen at –60oC to –80oC. Avoid repeated freeze/thaw of the samples.
  In-house turnaround time:5 days
  Reference Values:< 75 copies HIV-1 RNA / mL
  CPT Code:87536

Human Papiloma Virus (High Risk)  (Wilkinson, Edward J., MD Massoll, Nicole Ann, MD )

  Test Order Number:0813
  Methodology:Digene HC2
  Specimen Requirements:Pap specimen in Cytyc or SurePath solution, cervical biopsies in Digene STM solution
  Specimen Handling:Biopsies should be stored at -20°C, and can be shipped overnight at room temprature. The Pap solutions can be stored and shipped at room temperature.
  In-house turnaround time:5 days
  Reference Values:not detected
  CPT Code:87621

IA-2 Autoantibody  (William E. Winter, M.D.)

  Test Order Number:0278
  Methodology:RIA
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:< 0.76 u/mL
  CPT Code:86341

Immunoglobulin heavy chain gene rearrangement (B cell Clonality)  (Li, Ying, MD, PhD)

  Test Order Number:0256
  Methodology:PCR, DNA fragment analysis by 310
  Specimen Requirements:Tissue, cell suspension, peripheral blood in EDTA
  Specimen Handling:Ship blood or bone marrow specimens at 2-8oC. Ship Formalin fixed and/or paraffin embedded samples at room temperature.
  In-house turnaround time:7 days
  Reference Values:Polycolonal, negative
  CPT Code:83907, 83891, 83898x3, 83909x3, 83912x3

Insulin Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0018
  Methodology:RIA
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery
  In-house turnaround time:20 working days
  Reference Values:< 120 nU/mL
  CPT Code:86337

Islet Cell / Insulin Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0162
  Methodology:Indirect Immunofluorescence; RIA
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery
  In-house turnaround time:10 working days (see Notes)
  Reference Values:Negative (Quantitative results reported in JDF units)
  CPT Code:88347, 86337

Islet Cell Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0017
  Methodology:Indirect Immunofluorescence
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:Negative (< 5 JDF)
  CPT Code:88347

Methylenetetrahydrofolate Reductase (MTHFR) Mutation  (Dr. Neil Harris)

  Test Order Number:0276
  Methodology:PCR
  Specimen Requirements:5 mLs of anticoagulated blood in EDTA (purple top) collected by normal non-traumatic venipuncture.
  Specimen Handling:Store and ship at room temperature
  In-house turnaround time:10 days
  Reference Values:Normal
  CPT Code:83891, 83892, 83894, 83912, 83896, 83898

Microsatellite Instibility (MSI)  (Liu, Chen, MD, PhD Crawford, James M., MD, PhD )

  Test Order Number:MSI
  Methodology:PCR, DNA fragment analysis by 310
  Specimen Requirements:Formalin fixed and/or paraffin embedded samples
  Specimen Handling:ship and store at room temperature
  In-house turnaround time:9 days
  Reference Values:MSI-SS
  CPT Code:83907x2, 83890x 2, 83900 x 2, 83901 x 6, 83909 x 2

Neuropathology, Muscle Pathology, General Info.  (Anthony Yachnis, M.D.; Thomas Eskin, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemitry).
  Specimen Requirements:Tissues in appropriate fixative/preservative; pre-prepared slides and/or paraffin block. ***These specimens must be accompanied by x-rays.***
  Specimen Handling:
  In-house turnaround time:2 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Ovarian Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0039
  Methodology:Indirect Immunofluorescence
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery
  In-house turnaround time:10 working days
  Reference Values:Negative
  CPT Code:88347

Pap Smear, Conventional  (Nicole Massoll, M.D. Edward J. Wilkinson, M.D. Jacqueline A. Knapik, M.D. Anthony Yachnis, M.D. Demaretta Rush, M.D. )

  Test Order Number:
  Methodology:Conventional Cytologic Evaluation
  Specimen Requirements: Legibly labelled glass slides (patient first & last names, DOB); use pencil
  Specimen Handling:Interpretative Report
  In-house turnaround time:1 to 3 days
  Reference Values:
  CPT Code:Dependant on Testing Circumstances

Paternity Testing  (Scornik, Juan C., MD)

  Test Order Number:400-403
  Methodology:PCR, DNA fragments analysis
  Specimen Requirements:Buccal brush collection,
  Specimen Handling:store at 4oC
  In-house turnaround time:10 days
  Reference Values:
  CPT Code:83891x2, 83900x2, 83901x7x2, 83909x2, 83912x2

Placental (syncytrophoblast) Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0108
  Methodology:Indirect Immunofluorescence
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:Negative
  CPT Code:88347

Prothrombin  (Dr. Neil Harris)

  Test Order Number:0258
  Methodology:PCR
  Specimen Requirements:5 mLs of anticoagulated blood in EDTA (purple top) collected by normal non-traumatic venipuncture.
  Specimen Handling:Store and ship at room temperature.
  In-house turnaround time:10 days
  Reference Values:Normal
  CPT Code:83891, 83894 x 2, 83898 x 2, 83912

Pulmonary Pathology, General Info.  (Li Lu, M.D.Ph.D ; Peter A. Drew, M.D.; Thomas A. Eskin, M.D.; Nicole Massoll, M.D.; Robert Allan, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry).
  Specimen Requirements:Tissue in appropriate fixative/preservative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:2 to 5 days
  Reference Values:Interpretive report
  CPT Code:

Quantitative Pathology, General Info.  (Edward J. Wilkinson, M.D.; Peter A. Drew, M.D.; Nicole Massoll, M.D.)

  Test Order Number:
  Methodology:Computer-assisted morphometry for quantitative immunohistochemistry and flow cytometry analyses.
  Specimen Requirements:Paraffin block; formalin fixed tissue.
  Specimen Handling:
  In-house turnaround time:2 to 5 days
  Reference Values:Interpretive report with histogram
  CPT Code:

Renal Pathology, General Info.  (Byron P. Croker, M.D., Ph.D.; William L. Clapp, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, immunofluorescence (direct and indirect), special stains (conventional and immunohistochemistry).
  Specimen Requirements:Tissue in buffered formalin or other appropriate fixative for light microscopy and electron microscopy; Michel's media for immunofluorescence; pre-prepared slides; paraffin blocks.
  Specimen Handling:
  In-house turnaround time:4 to 10 days
  Reference Values:Interpretive report
  CPT Code:

Surgical Pathology, General Info.  (Christine Orlando, D.O.; Peter A. Drew, M.D.)

  Test Order Number:
  Methodology:Light microscopy, electron microscopy, special stains (conventional and immunohistochemistry), in situ hybridization, F.I.S.H..
  Specimen Requirements:Tissue in appropriate fixative/preservative; pre-prepared slides and/or paraffin block.
  Specimen Handling:
  In-house turnaround time:1 to 5 days
  Reference Values:Interpretive report
  CPT Code:

T cell Receptor γ Chain Gene Rearrangement  (Li, Ying, MD, PhD)

  Test Order Number:0252
  Methodology:PCR
  Specimen Requirements:Tissue, cell suspension, peripheral blood in EDTA
  Specimen Handling:Ship blood or bone marrow specimens at 2-8oC. Ship Formalin fixed and/or paraffin embedded samples at room temperature.
  In-house turnaround time:7 day
  Reference Values:Polycolonal, negative
  CPT Code:83907, 83891, 83900, 83901X2, 83894, 83912

Testicular Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0040
  Methodology:Indirect Immunofluorescence
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to plastic vial. Ship with frozen coolpack for overnight delivery
  In-house turnaround time:10 working days
  Reference Values:Negative
  CPT Code:88347

Thyroglobulin Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0020
  Methodology:Hemagglutination
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:Negative
  CPT Code:86800

Thyroid Microsomal Autoantibodies  (William E. Winter, M.D.)

  Test Order Number:0019
  Methodology:Hemagglutination
  Specimen Requirements:1-2 mL serum
  Specimen Handling:Transfer to a plastic vial. Ship with frozen coolpack for overnight delivery.
  In-house turnaround time:10 working days
  Reference Values:Negative
  CPT Code:86376

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